从cDNA文库中筛选分析阳性克隆的简便方法

cDNA文库中阳性克隆的传统筛选分析法既费时又费力.利用微波炉加热的方法,简化了原位杂交中噬菌斑裂解、DNA变性与固定的程序;进一步运用PCR扩增技术,特异扩增克隆载体中插入的cDNA片段,加快了阳性克隆分析的进程.     

滇藏常绿杜鹃亚属的修订

常绿杜鹃亚属Subgenus Hymenanthes是由K.Koch（1872）根据Blume（1826）建立的Genus Hymenanthes改级组合而成。但长期以来人们一直沿用了Maximowicz（1870）所建立的、后由Koehne（1893）修订过的Subgenus Eurhododendron这一亚属名称,直到最近D.F.Chamberlain（1978,1979,1982）对该亚属的名称才作了正式订正。     

Production of Ethanol from d-Xylose by Using d-Xylose Isomerase and Yeasts

d-Xylulose, an intermediate of d-xylose catabolism, was observed to be fermentable to ethanol and carbon dioxide in a yield of greater than 80% by yeasts (including industrial bakers' yeast) under fermentative conditions. This conversion appears to be carried out by many yeasts known for d-glucose fermentation. In some yeasts, xylitol, in addition to ethanol, was produced from d-xylulose. Fermenting yeasts are also able to produce ethanol from d-xylose when d-xylose isomerizing enzyme is present. The results indicate that ethanol could be produced from d-xylose in a yield of greater than 80% by a two-step process. First, d-xylose is converted to d-xylulose by xylose isomerase. d-Xylulose is then fermented to ethanol by yeasts.     

寡聚脱氧核苷酸d(G-C)_3的激光喇曼谱及其分析

用改进的固相磷酰三酯法合成了oligo-d(G-C)_3。以氩离子激光为激发光源,波长488nm.,在室温条件下,分别测定了纯化后的oligo-d(G-C)_3和其组分单体5’-dGMP和5’-dCMP的激光喇曼谱。观察到被测定的物质在300-2500cm~(-1)频率区间,各自都有其特征的谱形和喇曼峰。5’-dGMP和5’-dCMP谱中大多数特征峰在寡聚体的谱中消失,而在oligo-d(G-C)_3谱中出现了几处新的喇曼峰。经查证,峰832,851和899cm~(-1)系糖-磷酸主链的特征喇曼峰,另外几处峰与DNA的构象有关。实验结果表明oligo-d(G-C)_3在水溶液中(室温)主要以B-构象存在。     

通风结构林带背风面湍流相关特征初步分析

本文根据湍流统计理论和野外观测数据,详细分析了林带背风面湍流自相关、空间相关和湍流积分尺度特征。在林带背风面的林缘附近,由于湍流尺度较小,使湍流相关系数不紧密。这时的时间尺度在2—3s(1.5m)和3—8s(5m),而空间尺度为10—20m左右。在远离林带以后,林带的作用减弱,随着湍流尺度增大,导致湍流相关系数增大。这时的时间尺度在15—20s,空间尺度在70—80m范围内,且湍流自相关系数服从2/3的指数定律。     

东北主要造林树种的营养元素含量特征分析

 本文分析测定了东北地区9个主要针阔叶树种的营养元素含量和季节动态,并应用主成分分析和系统聚类分析方法,分析了各树种之间的营养元素相互关系。从而证明了N,P,Ca是植物重要的临界营养元素,Fe和Mn是表土中高度积累元素,树木对前者的吸收与土壤表土中含量有关,而对后者则表现相对的独立性和选择吸收能力。从营养元素的吸收与利用角度考虑,红松与胡桃楸混交,落叶松与水曲柳混交,硬阔叶树与软阔叶树混交,针阔叶树之间混交或白桦与其它各树种混交都是合理的。     

腐蹄病致病因子节瘤拟杆菌蛋白酶在大肠杆菌...

Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay.